Decreased Cry1Ac activation by midgut proteases associated with Cry1Ac resistance in Helicoverpa zea
نویسندگان
چکیده
منابع مشابه
Fitness costs associated with Cry1Ac-resistant Helicoverpa zea (Lepidoptera: Noctuidae): a factor countering selection for resistance to Bt cotton?
The heritability, stability, and fitness costs in a Cry1Ac-resistant Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) colony (AR) were measured in the laboratory. In response to selection, heritability values for AR increased in generations 4-7 and decreased in generations 11-19. AR had significantly increased pupal mortality, a male-biased sex ratio, and lower mating success compared with the...
متن کاملCharacterization of a Cry1Ac toxin-binding alkaline phosphatase in the midgut from Helicoverpa armigera (Hübner) larvae.
Midgut membrane-bound alkaline phosphatases (mALP) tethered to the brush border membrane surface by a glycosylphosphatidylinositol (GPI) anchor have been proposed as crucial for Cry1Ac intoxication. In the present work, two full-length cDNAs-encoding alkaline phosphatases in the midgut of Helicoverpa armigera larvae were cloned and named HaALP1 (GenBank accession no. EU729322) and HaALP2 (GenBa...
متن کاملAPN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
Lepidopteran midgut aminopeptidases N (APNs) are phylogenetically divided into eight clusters, designated as APN1-8. Although APN1 has been implicated as one of the receptors for Cry1Ac in several species, its potential role in the mode of action of Cry2Ab has not been functionally determined so far. To test whether APN1 also acts as one of the receptors for Cry1Ac in Helicoverpa zea and even f...
متن کاملDevelopment of bollworms, Helicoverpa zea, on two commercial Bollgard® cultivars that differ in overall Cry1Ac levels
Research was conducted to quantify the development of the corn earworm (= bollworm), Helicoverpa zea (Boddie), on two different transgenic cotton cultivars (DP 50B and NuCOTN 33B) that contained different levels of the Cry1Ac endotoxin from the soil bacterium, Bacillus thuringiensis Berliner. Using a field cage, an inverse relationship between the amount of Cry1Ac among cultivars versus the wei...
متن کاملProduction and characterization of Bacillus thuringiensis Cry1Ac-resistant cotton bollworm Helicoverpa zea (Boddie).
Laboratory-selected Bacillus thuringiensis-resistant colonies are important tools for elucidating B. thuringiensis resistance mechanisms. However, cotton bollworm, Helicoverpa zea, a target pest of transgenic corn and cotton expressing B. thuringiensis Cry1Ac (Bt corn and cotton), has proven difficult to select for stable resistance. Two populations of H. zea (AR and MR), resistant to the B. th...
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ژورنال
عنوان ژورنال: Pest Management Science
سال: 2018
ISSN: 1526-498X,1526-4998
DOI: 10.1002/ps.5224